CLARIFICATION THE SCIENFIC NAMES FOR SOME BAMBOO SPECIES (BAMBUSA Schreb.) IN VIETNAM CAUSED BY THE MORPHOLOGICAL VARIATION BASED ON trnL-trnF, psbA-trnH AND matK SEQUENCES

Abstract

In this study, three suitable nucleotide regions (trnLF/trnFR,  psbA3’f/trnH and matK19F/matKR) were used to decode nucleotide sequences for 19 samples of three species B. vulgaris Schrader ex Wendland cv. vittata McClure, B. vulgaris Schrader ex Wendland.cv. wamin McClure and  B. ventricosa  McClure, which needed to clarification the scientific name because the morphological variation in living conditions. Results were successfully amplified for all samples with sequence length of 1000 bp forthe  trnL-trnF, 680 for the  psbA-trnH and 1500 bp for  matK. Comparison of nucleotide sequences of three generegions shown the samples of the same species have similarity 100 %.  The level of nucleotide similarity between B. vulgaris Schrader ex Wendland cv. Vittata McClure,  B. vulgaris Schrader ex Wendland.cv. wamin McClure and  B. vulgaris spieces (coded EF137524 in Genbank) was 98.1 % for  trnL- trnF and 100 % for the  psbA-trnH. The level of nucleotide similarity between  B. ventricosa McClure and B. ventricosa(coded EF137524 in Genbank) was 99.0 % for psbA-trnH. The level of nucleotide similarity between  B. ventricosa  McClure and  B. tuldoides(coded GU063083 in Genbank) was 99.7 % for trnL-trnF, 100 % for psbA-trnH and matK gene region. The obtained results confirmed two bamboo species  B. vulgaris Schrader ex Wendland cv. Vittata McClure and B. vulgaris Schrader ex Wendland.cv.  wamin McClure are the same species B. vulgaris. B. ventricosa McClure was the same B.tuldoides species.