ASSESSMENT OF REGENT, SHERPA AND PEGASUS DEGRADING CAPACITY OF SOME ISOLATED BACTERIA
Abstract
The use of pesticides has become an integral part of modem agricultural techniques, but has
also been developed for domestic and governmental purposes. Increased use of these chemicals
might have caused (or is strongly suspected to create) environmental pollution with impact on
ecosystems or, possibly, human health. Pesticides are used widely in Vietnam with increasing quantity. According to reports of Plant Protection Institute, the use and abuse of pesticides with high toxicity and even prohobited ones in Vietnam lead to high residues of those in fruids and vegetables.
In nature, microorganisms are regarded as active factor in transformation cycle of chemical
elements and balance environment. Adaptation of indigenous soil microbial populations occurs
as a result of contact with xenobiotics. Many bacterial strains can degrade human made xenobiotics belonging to the genera Flavobacterium, Pseudomonas, Agrobacterium, Bacillus.
Detoxification of pesticides by bacterial enzymes became focus of many investigations owing to
it's efficacy and economization. The goal of this study was isolation and screening bacteria capable of decreasing Sherpa, Pegasus, Regent concentrations in order to develop a bacterial formula for treatment pesticide's residues in vegetables. Four bacterial strains have been selected for treatment of Sherpa and Regent and 3 strains for Pegasus. Strain RI had the highest activity against Sherpa, it eliminated 83.39% Sherpa in culture medium after 3 days. Strain SI6 reduced Regent up to 76.76% comparing with control after 3 days culture in minimal medium supplemented with 0.5 mg/ml Regent. Under the conditions used, strain NT64 removed 68,38% Pegasus in culture medium.
The received results showed that RI and SI6 had wider substrate spectrum than SI and PL