STUDY ON SELENIUM ACCUMULATION IN TURKEY TAIL FUNGUS (TRAMETES VERSICOLOR) BY USING INSTRUMENTAL NEUTRON ACTIVATION ANALYSIS
Abstract
Medicinal mushroom Kawaratake Trametes versicolor strain obtained from Chiba
University, Japan, was fermented at stationary Erlenmayer flasks 250 ml, containing PG media
with Se (10 ppm as selenate) and without Se (control) supplement, incubated at 32-34°C (room
temperature in HoChiMinh City). Selenium was added as dissolve selenate Na2Se04, in which Se
was enriched with ""Se. Under neutron fluxes in nuclear reactor '^Se will be activated into ''Se
emitted gamma rays (n,-/ reaction), recorded for calculations of Se contents in the samples.
Cultivation of Kawaratake on mixed substrates based sawdusts supplemented with Se by
injecting directly into center region of the substrate based on rubber tree sawdust. Analysis of Se
contents in fungal biomass harvested by using INAA with neutron flux at lO'-.cm'.s'' in Nuclear
Reactor at Dalat City, Vietnam. The results obtained with Trametes versicolor showed Se levels in mycelial biomass up to 600-1500 ppm, while in the control (without Se supplement), only trace of Se (1 ppm more or less) was found. It would be related to the researches on configurations of complex of polysaccharides and proteins in biomass with high bioactivities. Se would substitute S for some due structures and enhance their antioxydant activities.