ALTERATION OF ACTIVITY OF PROTEASE, LIPASE, AMYLASE IN SOYBEN SEED GERMINATED IN WATER DEFICIENT CONDITION

Abstract

Germination is initial process in plant life cycle. The first event in the germination sequence is imbibition and biochemolysis provision in seeds. In this state, hydrolizing enzymeplay an important role. In this research, soybean was hydroponically planted in distilled waterand in sucrose solution at 10,8% (equivalence 9 atm). During geminaton stage the activity ofprotease, lipase and amylase enzymes was measured at I, 3, 5 and 7 days after planting. Proteasein creased gradually and reached the peak at 7 days. The increase was much higher in planted indistilled water, and enzyme activity was changed differrently between the cultivars. Lipaseactivity had been increased since the gemination had started to 5th day, and decreased at 7th day.The activity of lipase in plants planted in high osmatic presure (in sucrose solution) wasincreased more readily and higher than those in plants in distilled water. Lipase activity altered differrently between cultivars that differred in their tolerance to drought. Amylase and lipaseactivity had the same tendency of the change:, increased from the 1st to 5th days and decreased at 7th day. Thus, the alteration of enzyme activity was differred in planted condition and cultivars