MEDIUM OPTIMIZATION FOR IMPROVEMENT OF VANCOMYCIN BIOSYNTHESIS BY STREPTOMYCES ORIENTALIS 4912

Abstract

In fermentation technology for antibiotic production, medium composition plays a crucialrole and particularly influences on the biosynthesis and accumulation of antibiotics. In this study, optimization of critical medium components was implemented in order to attain a higher yield of vancomycin biosynthesis by actinomycete Streptomyces orientalis 4912. On the basis of inoculum medium 48 and antibiotic fermentation medium MTll by 4912 strain, the effects of various carbon and nitrogen sources on vancomycin productivity were evaluated. From the results obtained, sucrose and pepton were determined the most effective on vancomycin production. Further optimization of sucrose- and pepton-proportion in MTI 1 medium by mathematically planned Box-Wilson approach revealed the maximal yield of vancomycin biosynthesis in the medium consisting of glucose 22 g.l-1; sucrose 34 g.l-1; peptone 6.8 g.l-1; NaCI 2.5 g.r and CaC03 2 g.l-1. The results of antibiotic fermentation after 120-hour in the original and optimized media showed that biomass and vancomycin production of the latter were enhanced by 46.6%) and 38.7%, respectively