Development of enterotoxin gene expression bacteria of enterotoxigenic Escherichia coli caused diarrheal disease in piglets

Abstract

The objective of this study was to create recombinant enterotoxin gene expression cells. The
genetic fusion of enterotoxin gene was amplified and attached into 24a(+) and pET32a(+) vector
and then transfored to BL21 successfully. PCR amplification and nucleotide sequence were used for
determination of genetic fusion of enterotoxin gene in BL21 strain. The recombinant proteins were
evaluated by SDS-PAGE and the immunoreactivity was characterized by Western blotting. The
studied results showed that nucleotide sequences of genetic fusion of enterotoxin genes were
consistent with nucleotide sequences of STa, STb and LT on GeneBank. The desired recombinant
protein was expressed in LB broth with 1mM IPTG substance as inducer.