Diversification of 18S rRNA gene of Balatidium coli caused diarrhea in post-weaning piglets in some Southern provinces Do Tien Duy, Nguyen Le Dinh Phuong, Le Vo Truong

Abstract

The objective of this study aimed at determining the diversification of 18S rRNA gene of
Balantidium coli caused diarrhea in the post-weaning piglets in the pig breeding farms of some
southern provinces. There were 20 B.coli strains isolated from 20 post-weaning piglets at 30-45
days old having typical diarrhea. Gene sequences of these bacteria strains were analysed and
compared. The studied result showed that similarity level of nucleotide sequences of the B.coli
strains in this study was 97.4 – 99.6%. The mutation in the gene segment included the replace
types, loss and inserting one or more than one nucleotides in comparison with the referent bacteria
strains (SP08-Spain). The result of analyzing phylogenetic tree indicated that the B.coli strains in
this study were divided into 2 groups (group A including the strains located in the same branch with
the Chinese, Japanese strains and group B consisting of the strains located in the same branch
with the Philippines and Spain strains). Especially, the B.coli strains collecting from Dong Nai and
Ben Tre province located in both group A and B