Isolation and selection of some useful microorganisms living in the pig intestine

Abstract

From the material samples collected in the intestine of pigs raising in Gia Lam district, Ha Noi City, 31 strains of microorganisms were isolated, of which, there were 6 yeast strains. 4 bacteria strains, signing AH3, AH4, H11, H9 strains and V1 yeast strain were selected, having probiotic properties. The results of studying biological characteristics showed that they belonged to the group of moist-loving microorganisms, grew well at low pH (2-5) and at the concentration of 3% bile salt, 4 bacteria strains were capable in inhibiting E.coli, Salmonella sp. The result of classification based on biological characteristics and sequence analysis of 16S rRNA genes of 4 bacteria strains showed that they presented high similarity level (> 99%) with species: Lactobacillus acidophilus, Bacillus licheniformis, Bacillus subtilis and the result of analyzing sequence of 5.8S rRNA gene of 1 yeast strain showed high similarity level (> 99%) with 1 species: Saccharomyces cerevisiae. All of 5 these strains belonged to the microorganism group that do not cause diseases for human and domestic animals. they can be used as the probiotics.