A geometrical optimisation of a coplanar micro-electrode structure for microfluidic flow cytometry

  • Van Phu Nguyen, Van-Anh Bui, Trung Kien Do, Van Thanh Pham, Thi Thanh Thuy Dang, Quang Loc Do*
  • Thu Hang Nguyen
Từ khóa: cell detection, impedance flow cytometry, microfluidic system, reactance, resistance

Tóm tắt

Single-cell analysis provides a more information-rich approach to disease diagnosis than traditional methods. At the cellular level, electrical properties have been established as reliable disease markers, capable of revealing variations between individual cells. This study focuses on optimising the geometry of a coplanar micro-electrode structure for detecting human lung adenocarcinoma cells (A549) within a fluid channel using impedance flow cytometry. A549
cells were chosen due to their frequent occurrence in cancer cases and the extensive documentation of their electrical properties and size. To further investigate the electric field and optimise the electrode design for single-cell detection, a numerical 3D model based on the finite element method (FEM) was developed and implemented. The functionality of the sensing structure was validated using COMSOL Multiphysics, with the Electric Current module defining scalar electric potential within the 3D model. Simulations explored various parameters, including electrode dimensions, frequency range, object sizes, and electrical conductivity, to fine-tune the sensor’s performance. Additionally, the study elucidates the impact of cell position within the channel structure and cell size on impedance measurements. This numerical investigation provides insights into the acquired impedance signals, contributing to the optimisation
and standardisation of the device. The proposed sensor system holds significant potential across various applications in biomedicine and chemistry, particularly in point-of-care scenarios, where the sensor chip can be conveniently configured for measurements and discarded after use. 

Tác giả

Van Phu Nguyen, Van-Anh Bui, Trung Kien Do, Van Thanh Pham, Thi Thanh Thuy Dang, Quang Loc Do*

Faculty of Physics, University of Science, Vietnam National University - Hanoi,
334 Nguyen Trai Street, Thanh Xuan Trung Ward, Thanh Xuan District, Hanoi, Vietnam

Thu Hang Nguyen

Faculty of Electronics and Telecommunications, University of Engineering and Technology, Vietnam National University - Hanoi,
144 Xuan Thuy Street, Dich Vong Hau Ward, Cau Giay District, Hanoi, Vietnam

điểm /   đánh giá
Phát hành ngày
2024-12-15
Chuyên mục
PHYSICAL SCIENCES