New distribution records of Rothmannia wittii (Rubiaceae) in Vietnam and identification of DNA barcode sequence for R. wittii

Abstract

Using morphological comparison method, the authors identified that the specimens collected in Binh Chau-Phuoc Buu Nature Reserve is Rothmannia wittii. Information on the distribution of this species in Vietnam was also reported. Moreover, based on molecular biology techniques, the matK and trnL-F regions of R. wittii were successfully amplified and sequenced. Also, the results showed that there were differences in matK and trnL-F sequences between R. wittii and R. daweishanensis that were similar in morphological characteristics.