PRESERVATION EFFICIENCY OF CATTLE STOOL BY VACUUM FOR DETERMNING INTESTINAL NEMATODE EGGS

Tóm tắt

Selecting an appropriate method for faecal preservation is one of the prerequisites for microscopy-based examination of parasitic eggs. This study was designed to evaluate the effectiveness of vacuum preservation technique for determination of nematode eggs in cattle faecal samples. Fecal samples were collected from 286 cattle and divided into two aliquots with each using different preservation methods. Simple flotation and McMaster technique were utilized to determine the prevalence and egg intensity (egg per gram), respectively. The results showed that the percentage of positive samples stored at vacuumed condition was 21.0% (95% CI: 16.4 - 26.2%) higher than those of 5% formalin with 13.3% (95% CI: 9.6 - 17.8%). The mean number of eggs per gram in samples that stored at vacuumed condition was 60 ± 44.5 higher than that of 5% formalin with 51.6 ± 33.4. Under vacuumed condition, simple flotation had a sensitivity of 92.3% (95% CI: 85.5 - 98.7%) higher than that of 5% formalin, 58.4% (95% CI: 41.51 - 70.4%). This study shows that faecal samples that kept at the condition of vacuum is superior to those preserved in 5% formalin for qualitative and quantitative purposes of nematode eggs in cattle feces.