BLOOD GLUCOSE-LOWERING, REGULATING ENZYME ACTIVITY OF GLUCOSE-6-PHOSPHATASE AND ANTIOXIDANT IN VIVO OF Nauclea orientalis L. ROOT EXTRACT

Abstract

The present study aimed to estimate the activities of malondialdehyde, glutathione and glucose-6-phosphatase in the pancreas, liver and kidneys of diabetic mice treated by extracts of Nauclea orientalis L. roots. In addition, this study evaluated the antioxidant activity and marker enzymes inhibitory (α-amylase; α-glucosidase) of the Nauclea orientalis L. roots extract. Hyperglycemia was induced in mice by administration of alloxan monohydrate (135 mg/kg, ip.). Hyperglycemic mice were treated with the extract of Nauclea orientalis L. roots (100, 200, 400 mg/kg body weight/ twice a day). Glucosephage was used as a possitive control agent with dose of 108 mg/kg body weight/twice a day. The experimental mice were administered with both Glucophage and Nauclea orientalis L. roots extract orally during 21 days. The treatment of Nauclea orientalis L. roots and Glucophage significantly decreased the blood glucose levels; suppressed the abnormal elevations of malondialdehyde levels and increased the activity of glutathione in the pancreas, liver and kidneys of diabetic mice. Besides, the results proved that the extracts of Nauclea orientalis L. roots also managed the excessive activity of glucose-6-phosphatase.