INVESTIGATION OF CELL PROLIFERATION INHIBITION THROUGH PROTEIN TP53-INDUCIBLE GLYCOLYSIS AND APOPTOSIS REGULATOR (TIGAR) OF COMPOUNDS ISOLATED FROM Goniothalamus elegans Ast

Abstract

Our study investigated the ability of Goniothalamus elegans Ast to inhibit growth of cancer cell, and evaluated the biological mechanism through inhibition of TIGAR protein activity. Extracts, fractions and isolated compounds were tested for the cytotoxicity of the HepG2 cancer cell line. The extract showed strong mode with IC₅₀ value of 19.74 µg/mL. Notably, the IC₅₀ values of two alkaloids, liriodenine and lysicamine were 18.12 ± 0.21 and 34.48 ± 1.21 µg/mL, respectively. The molecular docking method was used to validate the interaction between compounds and TIGAR proteins. The results indicate that liriodenine and lysicamine have strong binding affinity with TIGAR protein with values of -8.2 and -7.8 Kcal/mol, respectively. Our study shows that G. elegans extract and two compounds including liriodenine and lysicamine have the ability to inhibit the growth of HepG2 cancer cell line through the mechanism of binding to TIGAR protein. However, further experiments are required for confirming the molecular mechanisms through the TIGAR protein.