ISOLATION OF LACTIC ACID BACTERIA WITH ANTIFUNGAL ACTIVITY AGAINST Colletotrichum sp. CAUSING ANTHRACNOSE ON POSTHARVEST CAPSICUM FRUITS

Abstract

Anthracnose caused by Colletotrichum sp. appear more popular, causing much damage to the yield and quality of many crops. Therefore, this study was conducted to isolate and select lactic acid bacteria against Colletotrichum sp. causing anthracnose on postharvest capsicum fruits in Binh Tan district, Vinh Long province. As the results, 7 strains of lactic acid bacteria were isolated from traditional fermented products such as kimchi, sauerkraut, and sour shrimp. Antagonistic activity of isolated lactic acid bacteria against Colletotrichum sp. was carried out by antagonistic mycelia and spore methods. The results showed that 7/7 strains of lactic acid bacteria performed the antifungal activity Colletotrichum sp., including 6/7 strains with the well resistance and 1/7 strains with moderate resistance. KC2R strain was noted with the significantly highest antibacterial activity to Colletotrichum sp., with an antagonistic efficiency by the mycelium method of 25.40%, and average inhibition zone by spore method is 5.62 cm. Based on the results of morphological characteristics, the 16S rRNA gene sequencing showed that the KC2R strain were classified belonging to the species of Lactobacillus plantarum with 100% similarity. Research results showed that KC2R strain has the high application potential to post-harvest chili storage.