COMPARISION THE ANTIFUNGAL ACTIVITY AGAINST FUSARIUM OXYSPORUM OF 12 DIFFERENT ESSENTIAL OIL ENCAPSULATED IN NANOEMULSION AND THEIR COMBINATION

Abstract

The study aims to evaluate the in vitro antifungal activity against Fusarium oxysporum of 12 plant essential oils encapsulated nanoemulsion (EO-LNs) and their binary and ternary combined formulations of the 4 EOs having the highest in vitro antifungal activity. Firstly, 12 EO-LNs were prepared by a combining method of homogenization and sonication. Their binary and ternary combined formulations were also prepared by mixing with an equal volume ratio of each EO-LNs. In vitro antifungal activity of 12 EO-LNs and their combined formulations was evaluated using petri dish assay. The results indicated that 12 EO-LNs and their combined formulations were successfully prepared with the mean particle size at the nanoscale and the zeta potential greater than -42 mV. The results also showed that the combined formulations of EO-LNs had better antifungal activity against F. oxysporum compared to the corresponding individual EO-LNs. Specially, naTS exhibited the highest in vitro antifungal effect with the growth inhibition value of 88.14% at 500 times dilution. Therefore, naTS has a great potential application as an effective biofungicide against F. oxysporum.