STUDY ON ANTIBACTERIAL ACTIVITY AND ISOLATION OF STEROIDAL SAPONIN FROM THE ROOTS OF DRACAENA BRAUNII ENGL.

Abstract

Dracaena braunii Engl., synonym: Dracaena sanderiana, belongs to the Dracaena genus, thus the Asparagaceae family, is widely found in Africa and Asia. In Vietnam, this plant is distributed in natural place and also planted indoors for ornamental purpose. D. braunii is placed in the Dracanea genus which has known as an rich source of biological compounds. In this study, the antibacterial activity was carried out on the ethanolic extract of the roots of D. braunii. The result showed that the extracts at the concentration of 0.6, 1.0 and 1.4 g/mL can inhibit the growth of  S. aureus, P. aeruginosa, L. plantarum, E. coli and C. freundii, which highest at 1.4 g/mL and lowest at 0.6 g/mL. The chromatographical isolation was further carried out on the extract at the concentration of 1.4 g/mL affording one steroidal saponin. The spectroscopic analysis (NMR) and mass spectroscopy (ESI-MS) was performed on this compound, further establishing the structure as 26-O-β-D-glucopyranosyl-furost-5,25(27)-dien-1β,3β,22,26-tetrol 1-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside.