STUDY PROPAGATATION OF TECHTONA GRANDIS LINN (STRAINS VN34 and VN19) BY TISSUE CULTURE METHOD

Abstract

Study on the propagation of Tectona grandis L. f. (Teak) two strains VN34 and VN19 by the method of cultivating plant cell tissue, including the study: appropriate concentration of axillary bud disinfectant, growth stimulants to shoot multiplier, stimulation of Teak roots. The medium was used for the axillary buds of the teak strains VN34 and VN19 as MS*. As a result, on MS* medium, using disinfectant 0.1% HgCl2 for 10 minutes, obtained the best axillary bud quality (shoot multiplier - SM, shoot height - SH, ratio rooting- RR, root length - RL, average roots - AR). The growth stimulant added was a combination of BAP, NAA (0.75 mg/l BAP+0.25 mg/l NAA), which resulted in the best shoot multiplication compared to using BAP or Kinetin alone. The root stimulant is IBA was the most effective rooting rate with a concentration of 0.75 mg/l IBA.