CONTRUCTION VECTOR CONTAINING SALMONELLA TYPHIMURIUM LT2 STOXIC GENE DNA EXPRESSION IN E. COLI

Abstract

In vivo expression technology was used to the determine Salmonella typhimurium LT2 virulence gene. The result obtained that plasmid pCA 19 which contained toxic S. typhimurium LT2 gene was designed successfully. The expression of plasmid pCA19 in E .coli MGI655 wascarried out by reporter gene system. A reporter gene is a gene that encodes an assayable proteinwhose expression is easily detectable. In this study lacZ reporter gene was used to encode forenzyme galactosidase. This enzyme converts colorless substrate X - gal into galactose (colorless) and blue product. The result showed that some blue colonies appeared on containingmedium X-gal substrate. It indicated that they contained toxic S typhimurium LT2 gene vectorexpressed in E. coli MG1655