ANTIOXIDANT AND IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT FROM Oldenlandia capitellata Kuntze LEAVES IN INDOMETHACIN-INDUCED GASTRIC INFLAMMATION IN MICE

Abstract

Objective: This study evaluates the antioxidant and immunomodulatory effects of ethanol extract from Oldenlandia capitellata (EEOC) in a mouse model of indomethacin (IND)-induced gastric inflammation. Methods: Mice were treated with IND (45 mg/kg), omeprazole (20 mg/kg), or EEOC (100, 200, and 300 mg/kg). Key biomarkers of oxidative stress and immune response, including malondialdehyde (MDA), hydrogen peroxide (H₂O₂), glutathione (GSH), total antioxidant capacity (TAC), catalase (CAT), superoxide dismutase (SOD), white blood cell (WBC) counts, Nitroblue tetrazolium reduction (NBT), total immunoglobulin (TI), and phagocytic activity (PA), were assessed. Results: The IND group showed increased MDA (3.57 ± 0.03 nmol/mg protein) and H₂O₂ (2.15 ± 0.04 nmol/g tissue) levels (p < 0.05). EEOC treatment, particularly at 300 mg/kg, significantly reduced oxidative stress markers (MDA: 2.26 ± 0.01 nmol/mg, H₂O₂: 1.36 ± 0.02 nmol/g, p < 0.05) and restored antioxidant enzyme activities. EEOC also modulated immune responses by reducing WBC, NBT, TI, and PA counts while improving phagocytic activity. Conclusion: EEOC mitigates oxidative stress and modulates immune responses in gastric inflammation, suggesting its potential as a therapeutic agent for oxidative stress-related conditions.

DOIs: https://www.doi.org/10.46242/jstiuh.v74i2.5178