Ultrasound-Assisted Extraction of Inulin From Dandelion Leaves Application for Probiotics Spray Drying
DOI:
https://doi.org/10.54644/jte.2025.2016Tóm tắt
Dandelion leaves (Taraxacum officinale) are known for their high prebiotic inulin content. Ultrasound-assisted extraction (UAE) is considered one of the most efficient methods for improving extraction performance. This study focused on using an alternative ultrasound-assisted method to extract inulin from dandelion leaves. Additionally, the research explored the use of extracted inulin as a wall material in synbiotic spray drying with Lactobacillus acidophilus. Various combinations of hot water (70°C, 80°C, and 90°C) and ultrasonic power (30 W/g, 60 W/g, and 90 W/g) were tested for the extraction process. The material-to-water ratio was maintained at 1:10, with an extraction time of 20 minutes for all trials. The findings revealed that both water temperature and ultrasonic power had a significant impact on inulin concentration. The highest amount of extracted inulin (86.25 mg/g dw) was achieved with 60 W/g ultrasonic power at 80°C. UAE enhanced inulin extraction by 9.48% compared to traditional methods. The Lactobacillus acidophilus cells demonstrated satisfactory survival rates at high spray drying temperatures (inlet temperatures of 140°C and 180°C), indicating their heat resistance during spray drying. In conclusion, inulin extracted from dandelion leaves has promising potential as a wall material for spray-drying probiotics. However, further optimization of spray drying conditions is needed for efficient synbiotic production.
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[1] M. Shoaib et al., “Inulin: Properties, health benefits and food applications,” Carbohydrate Polymers, vol. 147, pp. 444–454, 2016.
[2] B. O. Srinameb, S. Nuchadomrong, S. Jogloy, A. Patanothai, and S. Srijaranai, “Preparation of inulin powder from Jerusalem artichoke (Helianthus tuberosus L.) tuber,” Plant Foods for Human Nutrition, pp. 221–226, 2015.
[3] M. B. Roberfroid, “Introducing inulin-type fructans,” Br. J. Nutr., 2005, doi: 10.1079/BJN20041350.
[4] S. Kolida and G. R. Gibson, “Prebiotic capacity of inulin-type fructans,” J. Nutr., 2007, doi: 10.1093/jn/137.11.2503S.
[5] N. Kaur and A. K. Gupta, “Applications of inulin and oligofructose in health and nutrition,” J. Biosci., pp. 703–714, 2002.
[6] Y. Moreno-Mendoza et al., “Effect of moringa leaf powder and agave inulin on performance, intestinal morphology, and meat yield of broiler chickens,” Poultry Sci., vol. 100, no. 2, pp. 738–745, 2021.
[7] A. Bhanja, P. P. Sutar, and M. Mishra, “Inulin—A polysaccharide: Review on its functional and prebiotic efficacy,” J. Food Biochem., vol. 46, no. 12, p. e14386, 2022.
[8] L. G. Ooi, R. Bhat, A. Rosma, K. H. Yuen, and M. T. Liong, “A synbiotic containing Lactobacillus acidophilus CHO-220 and inulin improves irregularity of red blood cells,” J. Dairy Sci., vol. 93, no. 10, pp. 4535–4544, 2010.
[9] L. Cai, D. Wan, F. Yi, and L. Luan, “Purification, preliminary characterization and hepatoprotective effects of polysaccharides from dandelion root,” Molecules, vol. 22, no. 9, p. 1409, 2017.
[10] H. Guo et al., “Physicochemical, structural, and biological properties of polysaccharides from dandelion,” Molecules, vol. 24, no. 8, p. 1485, 2019.
[11] M. González-Castejón, F. Visioli, and A. Rodriguez-Casado, “Diverse biological activities of dandelion,” Nutr. Rev., vol. 70, no. 9, pp. 534–547, 2012.
[12] L. De Leenheer, “Production and use of inulin: Industrial reality with a promising future,” Carbohydrates as Organic Raw Materials, pp. 67–92, 1996.
[13] S. Li et al., “Development of a combined trifluoroacetic acid hydrolysis and HPLC-ELSD method to identify and quantify inulin recovered from Jerusalem artichoke assisted by ultrasound extraction,” Appl. Sci., 2018, doi: 10.3390/app8050710.
[14] M. Du et al., “Extraction, physicochemical properties, functional activities and applications of inulin polysaccharide: A review,” Plant Foods Hum. Nutr., vol. 78, no. 2, pp. 243–252, 2023.
[15] F. Chemat and M. K. Khan, “Applications of ultrasound in food technology: Processing, preservation and extraction,” 2011, doi: 10.1016/j.ultsonch.2010.11.023.
[16] Z. Ying, X. Han, and J. Li, “Ultrasound-assisted extraction of polysaccharides from mulberry leaves,” 2011, doi: 10.1016/j.foodchem.2011.01.083.
[17] N. T. M. Le and N. T. Huong, “Microencapsulation of Lactobacillus fermentum 39-183 by spray drying in the presence of prebiotics,” J. Sci. Food Technol., pp. 11–20, 2017.
[18] B. M. Corcoran, R. P. Ross, G. F. Fitzgerald, and C. Stanton, “Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances,” J. Appl. Microbiol., vol. 96, no. 5, pp. 1024–1039, 2004.
[19] R. P. Ross, C. Desmond, G. F. Fitzgerald, and C. Stanton, “Overcoming the technological hurdles in the development of probiotic foods,” J. Appl. Microbiol., vol. 98, no. 6, pp. 1410–1417, 2005.
[20] N. Petkova, G. Sherova, and P. Denev, “Characterization of inulin from dahlia tubers isolated by microwave and ultrasound-assisted extractions,” Int. Food Res. J., vol. 25, no. 5, pp. 1876-1884, 2018.
[21] W. O. Noori, “Selection of optimal conditions of inulin extraction from Jerusalem artichoke (Helianthus tuberosus L.) tubers using ultrasonic water bath,” J. Eng., pp. 110–119, 2014.
[22] S. Zhao, K. C. Kwok, and H. Liang, “Investigation on ultrasound-assisted extraction of saikosaponins from Radix Bupleuri,” Sep. Purif. Technol., vol. 55, no. 3, pp. 307–312, 2007.
[23] R. Jovanovic-Malinovska, S. Kuzmanova, and I. Winkelhausen, “Application of ultrasound for enhanced extraction of prebiotic oligosaccharides from selected fruits and vegetables,” Ultrason. Sonochem., vol. 22, pp. 446–453, 2015.
[24] L. G. d’Alessandro, K. Kriaa, I. Nikov, and K. Dimitrov, “Ultrasound-assisted extraction of polyphenols from black chokeberry,” Sep. Purif. Technol., vol. 93, pp. 42–47, 2012.
[25] H. Koiwai and N. Masuzawa, “Extraction of catechins from green tea using ultrasound,” Jpn. J. Appl. Phys., vol. 46, no. 7S, p. 4936, 2007.
[26] P. Simpson et al., “Intrinsic tolerance of Bifidobacterium species to heat and oxygen and survival following spray drying and storage,” J. Appl. Microbiol., vol. 99, no. 3, pp. 493–501, 2005.
[27] C. Saénz et al., “Microencapsulation by spray drying of bioactive compounds from cactus pear (Opuntia ficus-indica),” Food Chem., vol. 114, no. 2, pp. 616–622, 2009.
[28] S. Arslan, M. Erbaş, İ. Tontul, and A. Topuz, “Microencapsulation of probiotic Saccharomyces cerevisiae var. boulardii with different wall materials by spray drying,” LWT—Food Sci. Technol., vol. 63, no. 1, pp. 685–690, 2015.