A STUDY ON ESTABLISHING ANALYTICAL CHEMISTRY PRACTICE ON UHPLC-PDA/MS EQUIPMENT

Tóm tắt

In this study, an analytical chemistry practice was established and validated via the quantification of ibuprofen in drugs using the UHPLC-PDA/MS analysis for undergraduate students. Accordingly, ibuprofen, extracted from the drug powder, was determined by Acquity UPLC® BEH C18 column (1.7 μm; 2.1 × 50 mm) equipment with a mixture of 0.1% HCOOH:ACN (40:60, v/v) as a mobile phase, and an isocractic flow rate of 0.9 mL min‒1 at a quantitative wavelength of 254 nm. The data show that the analytical method used possesses high specificity, good linear correlation, and high system suitability. Accordingly, the repeatability (RSD), recovery, limit of detection (LOD), and limit of quantification (LOQ) for this method are 0.75%, 101.3%, 1.00 ppm, and 3.3 ppm, respectively. In particular, the results of the study indicate that the practice is performed at intervals of 4-4.5 hours, with the analytical process reaching the requirements of intermediate precision. In addition, the parameters were further confirmed by the detector of mass spectroscopy (MS) incorporated with the UHPLC device. These findings demonstrate that the analytical procedure is a promising and effective method for establishing the analytical chemistry practice to meet the general education curriculum 2018 in Vietnam and serve as a valuable reference for students and teachers