TWO NEW STRAINS OF MICROALGAE Scenedesmus sp. RECENTLY ISOLATED AND IDENTIFIED BY 18S SEQUENCING FROM THE CAN GIO MANGROVE BIOSPHERE RESERVE

Tóm tắt

The microalgae Scenedesmus, belonging to the Chlorophyta phylum, is a widely distributed freshwater algae species. Many species from this genus are found in the Can Gio mangrove biosphere reserve, where they play a significant role in the aquatic environment. In this study, two microalgae strains, Scenedesmus CG01 and CG03, were newly isolated from two different sampling sites within the Can Gio mangrove biosphere reserve. These two strains exhibit similar morphological features typical of the genus Scenedesmus. PCR amplification, sequencing, and identification based on the 18S DNA sequence confirmed that both strains, CG01 and CG03, belong to Scenedesmus sp. The 18S DNA sequences of these strains differ from those of Scenedesmus sp. CCAP 217/7 and CCAP217/8 in GenBank. The phylogenetic analysis of the 18S sequences revealsthat CG01 and CG03 are in a distinct cluster, separate from the remaining samples. This research on the isolation and accurate identification of species provides an important foundation for future studies on the application of microalgae.