A validated hplc-elsd procedure for the simultaneous determination of major saponins in lai chau ginseng (panax vietnamensis var. Fuscidiscus)

Tóm tắt

Panax vietnamensis var. fuscidiscus, commonly known as Lai Chau ginseng, is a valuable variety of Vietnamese ginseng whose chemical characteristics remain insufficiently documented. This study aimed to develop and validate a quantitative method using high-performance liquid chromatography coupled with an evaporative light scattering detector to simultaneously determine nine characteristic saponins in Lai Chau ginseng. Extraction conditions and chromatographic parameters were optimized to improve analytical efficiency. The validated method demonstrated strong system suitability, excellent linearity (R² > 0.99), high precision with relative standard deviations below 2%, and acceptable accuracy with recoveries ranging from 90% to 110%. The study provides essential quantitative data that support future efforts toward establishing a standardized quality control framework for this medicinally and economically important Vietnamese ginseng variety.