Evaluation of the hydrolysis efficiency of shortfin scad (Decapterus russelli) using alcalase for the production of soluble protein hydrolysate rich in amino acids
Abstract
The research has been conducted on the hydrolysis process of Indian scad (Decapterus russelli) protein using Alcalase enzyme under natural pH conditions and a water/material ratio of 1/1. The results show that in order to achieve optimal efficiency, it is necessary to use an enzyme-substrate ratio of 93.48 AU/kg protein, to maintain a temperature of 60ºC for 6 hours. Following this process, the highest hydrolysis degree achieved was 73.01%, with a nitrogen recovery efficiency of 75.30% and a total volatile basic nitrogen of 0.8 g/l in the obtained hydrolysate solution. The final product contains 13.45 g/l of amino acid nitrogen and 0.59 mg/100 g of histamine, demonstrating a significant utilisation for diverse applications across various fields.