HISTOLOGICAL CHARACTERISTICS AND IMMUNE RESPONSE OF ATOPIC DERMATITIS INDUCED BY TOPICAL APPLICATION OF 2,4-DINITROCHLOROBENZENE IN SWISS ALBINO MICE

Abstract

Atopic dermatitis (AD) is a common inflammatory skin disorder with complex etiologies. Since there is no satisfactory therapy for AD treatment, the development of animal models provides a better understanding of AD pathogenesis. Induction of AD-like symptoms by 2,4-Dinitrochlorobenzene (DNCB) has been conducted in different mouse strains such as SKH1, BALB/c, C57BL/6 và Nc/Nga. However, such data is limited in Swiss albino mice. This study aimed to characterize the alteration of skin histology and immune responses in Swiss albino mice with AD-like symptoms induced by topical application of DNCB. Following the induction, pathological parameters including skin thickness, immunoglobulin (Ig) E level and mast cell infiltration were then measured. Histopathological analysis showed that topical application of DNCB exerted epidermal and dermal thickening and increased mast cell infiltration in the dermis. The serum level of IgE was also elevated in DNCB-treated mice.