EFFECT OF SOME FACTORS TO PROTEIN SOLUTION YIELD FROM THE Cordyceps militaris SPENT USING PAPAIN

Abstract

The Cordyceps militaris spent after receiving fruit body processing is a source of high nutrient by-product that has not been effectively reused. In this study, the effect of some factors on the protein solution yield was determined and optimized. From that, the papain activity and hydrolytic time were determined to enhance the protein solution yield. At the mushroom spent ratio of 7.5%, the optimized result by response surface methodology showed the highest protein content of 20.3 ± 1.2 mg/mL at 54 oC for 20 hours. Adding papain activity of 0.3 U/g mushroom spent for 3 hours helps enhance the protein content in the extract solution, reaching 24.4 ± 0.6 mg/mL. The result showed the potential extraction protein from C. militaris spent to produce rich nutrient food drinks