STUDY ON MORPHOLOGY, ANATOMY AND DISINFECTION METHOD TO PREPARE CLEAN EXPLANTS FOR IN VITRO PROPAGATION OF Adinandra sp.
Abstract
Genus Adinandra is a medicinal plant containing a wide range of pharmacologically active compounds that can be used in anti-inflammatory, antioxidant, prevention and treatment of cancer. This plan nowaday can be found distributionin some limitedareas of provinces such as Lao Cai, Vinh Phuc, Quang Tri, Ha Giang, Lam Dong. Recently, Adinandra is listed in the Viet Nam’s Red list of threatened species. The only effective way of artificial propagation to preserve and develop this genetic resource is the tissue culturing method. This study on the geographic distribution, morphological and anatomical conditions of the Adinandra sp. found in Liem Phu commune, Van Ban district, Lao Cai province, Vietnam. In this study, Adinandra is successfully propagated in vitro by using seeds as starting materials. These materials are sterilized using different chemical substances at different periods of time. After sterilizing materials in ethanol 70° for 1 minute then in HgCl2 0.1% solution for 12 minutes gives us the best performance of making clean samples. The disinfected samples then are cultivated in MS medium, clean samples proportion reaches its value 91%, the percentage of shoots generated 87.33% after 6 weeks of cultivating, the shoots are plump, deep green and have good quality.
