APPLICATION OF BIOFILM FORMING BACTERIA ON FINE SAWDUST AS A BIO-PRODUCT FOR TREATING OIL – CONTAMINATED WATER
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DOI:
https://doi.org/10.34238/tnu-jst.14741Abstract
Recently, the use of biofilm-forming bacteria on carriers to remove oil pollution has drawn increasing research interest. In this study, two biofilm-forming bacterial strains identified as Bacillus sp. B1 and Rhodococcus sp. B3 were isolated from the oil-contaminated samples taken from coastal zone in Thanh Hoa province. Both strains exhibited good growth in the presence of 250 ppm of a mixture of polycyclic aromatic hydrocarbons consisting of anthracene, pyrene and phenanthrene; as well as 300 ppm diesel oil. The strains formed stable biofilm at temperatures ranging from 25 to 45 oC, NaCl concentrations from 0 to 2% and pH values among 3 and 8. A bio-product was subsequently prepared by immobilizing the selected strains onto fine sawdust as a carrier material. The product degraded > 85 and 82% of the total diesel oil and mixture aromatic hydrocarbons in wastewater with an initial concentration of 250 and 200 ppm after 7 day-incubation, respectively. The viable cell density of the bio-product was 21x107 and 5x107 CFU/g at the beginning and after 6 months of preservation. In addition, fine sawdust is an abundant, inexpensive and biodegradable raw material. These findings may lead to the potential application of this bio-product for the oil pollution treatment in Vietnam.Downloads
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