QUANTITATIVE ANALYSIS OF ACTEOSIDE AND JIONOSIDE B1 IN THE TUBEROUS ROOTS OF Rehmannia glutinosa BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
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DOI:
https://doi.org/10.34238/tnu-jst.15257Abstract
This study reports the quantitative determination of two phenylpropanoid glycosides, Acteoside and Jionoside B1, in the tuberous roots of Rehmannia glutinosa using high-performance liquid chromatography (HPLC). The analysis was carried out on an Agilent 1260 Infinity II system equipped with a Hydrosphere C18 column and a diode-array detector (DAD) at 198 nm. Chromatographic conditions were optimized to ensure effective separation and accurate quantification of the target compounds. Calibration curves exhibited good linearity over the concentration range of 100–5000 μg/mL with correlation coefficients (R²) above 0.996. The validated method showed satisfactory precision, accuracy, and sensitivity in accordance with AOAC guidelines. The contents of Acteoside and Jionoside B1 in the total methanolic extract were 2.12% and 2.39%, respectively, corresponding to 0.59% and 0.66% in the dried tuberous roots of Rehmannia glutinosa. These results suggest that both compounds may serve as suitable chemical markers for the quality control and standardization of Rehmannia glutinosa and related medicinal products.Downloads
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