ISOLATION, SCREENING OF NITROGEN FIXING BACTERIA AND EVALUATION OF THEIR EFFECT ON MAIZE
Keywords
DOI:
https://doi.org/10.34238/tnu-jst.12807Abstract
In this study, two nitrogen fixing bacterial strains (T22 and T27) were isolated and selected from garden soil samples collected in Hoai Duc, Hanoi. The highest NH4+ concentrations synthesized by strains T22 and T27 were 24.9 g/L and 25.3 g/L, respectively. Strain T22 is a Gram-positive, rod-shaped bacterium, and able to produce catalase; strain T27 is a Gram-negative, short-rod-shaped bacterium, and able to produce catalase and decompose insoluble phosphate. The analysis of 16S rDNA sequences showed that strain T22 is closely related to species of the genus Bacillus and strain T27 belongs to the species Azotobacter chroococcum. Both selected bacterial strains are capable of using glucose, mannitol or molasses as carbon source, growing well at 30 oC and neutral pH. The two bacterial strains T22 and T27 showed positive effects on the growth of maize plant after four weeks of experiment. The results of the study showed that the two selected bacterial strains (Bacillus sp. T22 and Azotobacter chroococcum T27) holding the potential to produce biofertilizers to promote plant growth.Downloads
References
T. Searchinger, R. Waite, C. Hanson, J. Ranganathan, P. Dumas, E. Mathews, and A. Klirs, “Creating a sustainable food future: a menu of solutions to feed nearly 10 billion people by 2050,” World Resources Reports. World Resources Institute, 2019.
S. E. Bloch, M-H. Ryu, B. Ozaydin, and R. Broglie, “Harnessing atmospheric nitrogen for cereal crop production,” Current Opinion in Biotechnology, vol. 62, pp. 181-188, 2020.
K. Mahmud, S. Makaju, R. Ibrahim, and A. Missaoui, “Current progress in nitrogen fixing plants and microbiome research,” Plants, vol. 9, no. 1, Jan. 2020, Art. no. 97.
S. Ranjan, S. Sow, S. R. Choudhury, S. Kumar, and M. Ghosh, “Biofertilizer as a novel tool for enhancing soil fertility and crop productivity: a review,” International Journal of Current Microbiology and Applied Sciences, vol. S11, pp. 653-665, 2020.
P. Chaudhary, S. Singh, A. Chaudhary, A. Sharma, and G. Kumar, “Overview of biofertilizers in crop production and stress management for sustainable agriculture,” Frontiers in Plant Science, vol. 13, Aug. 2022, Art. no. 930340.
S. A. Wani, S. Chand, M. A. Wani, M. Ramzan, and K. R. Hakeem, “Azotobacter chroococcum–a potential biofertilizer in agriculture: an overview,” in Soil Science: Agricultural and Environmental Prospectives, K. R. Hakeem, J. Akhtar, M Sabir, Ed., Springer, 2016, pp. 333-348.
T. A. Bhat, L. Ahmad, M. A. Ganai, S. U. Haq, and O. A. Khan, “Nitrogen fixing biofertilizers; mechanism and growth promotion: a review,” Journal of Pure and Applied Microbiology, vol. 9, no. 2, pp. 1675-1690, 2015.
F. Kawaka, “Characterization of symbiotic and nitrogen fixing bacteria,” AMB Express, vol. 12, Jul. 2022, Art. no. 99.
A. Aasfar et al., “Nitrogen fixing Azotobacter species as potential soil biological enhancers for crop nutrition and yield stability,” Frontiers in Microbiology, vol. 12, Feb. 2021, Art. no. 628379.
S. Sun, Y. Chen, J. Cheng, Q. Li, Z. Zhang, and Z. Lan, “Isolation, characterization, genomic sequencing, and GFP-marked insertional mutagenesis of a high-performance nitrogen-fixing bacterium, Kosakonia radicincitans GXGL-4A and visualization of bacterial colonization on cucumber roots,” Folia Microbiologica, vol. 63, no. 6, pp. 789–802, 2018.
H. Mukhtar, H. Bashir, A. Nawaz, and I. Haq, “Optimization of growth conditions for Azotobacter species and their use as biofertilizer,” Journal of Bacteriology and Mycology, vol. 6, no. 5, pp. 274-278, 2018.
J. G. Cappuccino and C. Welsh, Microbiology: a laboratory manual 51-142, Pearson, 2020.
H. Heuer, M. Krsek, P. Baker, K. Smalla, and E. M. H. Wellington, “Analysis of actinomycete communities by speci¢c ampli¢cation of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients,” Applied and Environmental Microbiology, vol. 63, no. 8, pp. 3233-3241, 1997.
K. Tamura, G. Stecher, and S. Kumar, “MEGA 11: molecular evolutionary genetics analysis version 11,” Molecular Biology and Evolution, vol. 38, no. 7, pp. 3022-3027, 2021.
L. Zhou and C. E. Boyd, “Comparison of Nessler, phenate, salicylate and ion selective electrode procedures for determination of total amminia nitrogen in aquaculture,” Aquaculture, vol. 450, pp. 187-193, 2016.
T. Q. Ha and T. T. H. Chu, “Selection of nitrogen fixation and phosphate solubilizing bacteria from cultivationg soil samples of Hung Yen province in Vietnam,” Journal of Vietnamese Environment, vol. 12, no. 2, pp. 162-168, 2020.
S. A. Dar, R. A. Bhat, M. A. Dervash, Z. A. Dar, and G. H. Dar, “Azotobacter as biofertilizer for sustainable soil and plant health under saline environmental condition,” in Microbiota and Biofertilizers, K. R. Hakeem et al., (eds.), Springer Nature Switzerland AG, 2021, pp. 231-254.
