STUDY ON THE SKINBRIGHTENING AND ANTIOXIDANT ACTIVITIES OF EXTRACT FROM THE HUSK OF CASTANEA MOLLISSIMA BLUME IN VIETNAM
Keywords
DOI:
https://doi.org/10.34238/tnu-jst.13604Abstract
The study aimed to evaluate the tyrosinase enzyme inhibition and antioxidant effects of the total extracts from various parts and fractionated extracts: n-hexane, ethyl acetate, and methanol from the husk of the Castanea mollissima Blume collected in Trung Khanh district, Cao Bang province, using the 1,1-diphenyl-2-picrylhydrazylfree radical scavenging method and the tyrosinase enzyme inhibition assay. The results indicated that the total extract from the husk exhibited the strongest tyrosinase enzyme inhibitory activity, with an IC50 value of 325.43 ± 14.78 μg/mL. Meanwhile, the leaf, stem bark, and twig extracts showed moderate enzyme inhibition, with IC50 values ranging from 336.43 to 406.42 µg/mL. In the in vitro antioxidant activity test, the ethyl acetate extract demonstrated the best antioxidant activity with a scavenging capacity of 74.14 ± 0.4%, corresponding to an SC50 value of 18.76 µg/mL, while the n-hexane extract showed a free radical scavenging capacity of 60.49 ± 1.1%, corresponding to an SC50 value of 51.32 µg/mL. The other extracts displayed moderate to weak activity. This shows that the classes of substances exhibiting anti-free radical activity are mostly found in the less polar and moderately polar extracts.Downloads
References
T. B. Nguyen, The catalog of Vietnamese plant species, Hanoi: Hanoi Agriculture Publishing House; 2003, pp. 227-269.
T. T. L. Vu, S. L. Wang, T. T. T. Ho, Q. T. Luc, T. Q. Phan, T. K. T. Phan, T. B. H. Dam, T. K. P. Phan, A. D. Nguyen, and V. B. Nguyen, “Elucidation of potent mammalian enzymes inhibitors targeting anti‑diabetes drug from Castanea mollissima blume, 1851: an edible herbal collected in Vietnamvia experimental and computation approaching,” Res. Chem. Intermed, vol. 50, pp. 6065–6086, 2024.
T. T. Phung, D. X. Tran, T. K. Do, A. Ahmad, V. Q. Nguyen, T. T. A. Truong, H. A. La, and N. M. Truong, “Phenolic compositions and antioxidant properties in bark, flower, inner skin, kernel and leaf extracts of Castanea crenata Sieb. etZucc,” Antioxidants (Basel), vol. 6, pp. 31-46, 2017.
F. Yang, Q. Liu, S. Pan, C. Xu, and Y. L. Xiong, “Chemical Composition and Quality Traits of Chinese Chestnuts (Castanea mollissima) Produced in Different Ecological Regions,” Food Bioscience, vol. 11, pp. 33-42, 2015.
A. Kremer, A. G. Abbott, J. E. Carlson, P. S. Manos, C. Plomion, P. Sisco, M. E. Staton, S. Ueno, and G. G. Vendramin, “Genomics of Fagaceae,” Tree Genetics and Genomes, vol. 8, pp. 563-610, 2012.
L. Zhang, H. Y. Gao, M. Baba, Y. Okada, T. Okuyama, L. J. Wu, and L. B. Zhan, “Extracts and Compounds with Anti-Diabetic Complications and Anti-Cancer Activity from Castanea mollissina Blume (Chinese chestnut) ,” BMC Complementary and Alternative Medicine, vol. 14, no. 1, pp. 1-9, 2014.
Q. L. Le, L. H. H. Nguyen, V. M. Le, B. C. Phung, and H. D. Nguyen, “Depigmenting effect of Sophora japonica L. in b16f10 melanoma cells,” Journal of Science Technology and Food, vol. 17, no. 1, pp. 14-20, 2018.
J. G. Vaughan and C. A. Geissler, The New Oxford Book of Food Plants, 2nd edition. Oxford University Press, New York/Oxford, 1997.
T. Ozcan, L. Yilmaz-Ersan, A. Akpinar-Bayizit, and B. Delikanli, “Antioxidant Properties of Probiotic Fermented Milk Supplemented with Chestnut Flour (Castanea sativa Mill.),” J. Food Process Preserv., vol. 41, no. 5, 2017, Art. no. e13156.
Y. Lim, J.-W. Park, O.-K. Kwon, J.-W. Lee, H.-S. Lee, S. Lee, S. Choi, W. Li, H. Jin, S.-B. Han, “Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264. 7 macrophage cells,” International Journal of Molecular Medicine, vol. 41, pp. 391-398, 2014.
S. H. Han, M. S. Hur, M. J. Kim, W. H. Jung, M. Park, J. H. Kim, H. J. Shin, Y. B. Choe, K. J. Ahn, Y. W. Lee, “In vitro anti-Malassezia activity of Castanea crenata shell and oil-soluble Glycyrrhiza extracts,” Annals of Dermatology, vol. 29, pp. 321-326, 2017.
G. Squillaci, F. Apone, L. M. Sena, A. Carola, A. Tito, M. Bimonte, A. De Lucia, G. Colucci, F. La Cara, and A. Morana, “Chestnut (Castanea sativa Mill.) Industrial Wastes as A Valued Bioresource for The Production of Active Ingredients,” Process Biochem., vol. 64, pp. 228-236, 2018.
A. Basile, S. Sorbo, S. Giordano, L. Ricciardi, S. Ferrara, D. Montesano, C. R. Castaldo, M. L. Vuoto, and L. Ferrara, “Antibacterial and Allelopathic Activity of Extract from Castanea sativa Leaves,” Fitoterapia, vol. 71, pp. 110-116, 2000.
P. Comandini, M. J. Lerma-García, E. F. Simó-Alfonso, and T. G. Toschi, “Tannin Analysis of Chestnut Bark Samples (Castanea sativa Mill.) by HPLC-DAD-MS,” Food Chem., vol. 157, pp. 290-295, 2014.
M. Brenner and V. J. Hearing, “The protective role of melanin against UV damage in human skin,” Photochem. Photobiol., vol. 84, no. 3, pp. 539–549, 2008.
A. Hering, M. Gucwa, B. Wielgomas, and J. R. Ochocka, “Photoprotection and antiaging activity of extracts from Honeybush (Cyclopia sp.)—in vitro wound healing and inhibition of the skin extracellular matrix enzymes: tyrosinase, collagenase, elastase and hyaluronidase,” Pharmaceutics, vol. 15, no. 5, 2023, Art. no. 1542.
W. W. Brand et al., "Use of a Free Radical Method to Evaluate Antioxidant Activity,” Lebenm Wissu Technol, vol. 2, no. 1, pp. 25-30, 1995.
S. Gorinstein et al., "Comparison of the contents of the main biochemical compounds and antioxidant activity of some Spanish olive oils as determined by four different radical scavenging tests,” Journal of Nutritional Biochemistry, vol. 14, pp. 154-159, 2003.
A. Kumar et al., "Antioxidant Activity and Pharmacological Screening of Tinospora Cordifolia (Thunb.)," The Bioscan, vol. 8, no. 2, pp. 689-693, 2013.
