Method development for simultaneous analytical method for ten ginsenosides in soft-gel dietary supplements containing Panax spp. by LC-MS/MS

Tóm tắt

A liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantification of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3, notoginsenoside R1 and majonoside R2 in dietary supplement products containing ginseng. A Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) was employed as the separation column and maintained at 40℃ during analysis. The mobile phase consisted of 0.1% formic acid and acetonitrile, delivered under an optimized gradient program. Detection was by tandem mass spectrometry using electrospray ionization in the positive ion mode and multiple reaction monitoring (MRM). The sample were defatted with hexane before extraction with 50% methanol at 75℃ for 30 min by sonication. The method was validated following AOAC guidelines and Commission Decision 2002/657/EC. The results demonstrated good specificity, linearity over the range of 100 - 2000 ng/mL, the detection limit and quantitative limit of the method are 1.43 - 1.85 and 4.18 - 5.41 μg/g. The validated method was successfully applied to determine ginsenoside content in ten different ginseng-based dietary supplements available on the Vietnamese market.