Evaluation of the bacterial detection capability of the LuciPac™ A3 Surface kit using the Lumitester Smart system for surface hygiene monitoring

Tóm tắt

Surface hygiene monitoring is essential for controlling microbial contamination in food processing environments, yet conventional culture-based methods are time-consuming and unsuitable for rapid on-site assessment. The LuciPac™ A3 Surface method combined with the Lumitester Smart system enables rapid detection of total adenylate (ATP, ADP, and AMP) as an indicator of biological contamination. This study evaluated the detection capability and repeatability of the A3 method in comparison with conventional microbiological methods using representative ATCC strains. The A3 method detected most microorganisms at approximately 10⁵ CFU/mL, with lower detection limits observed for spore-forming bacteria, including Bacillus cereus (2.2 x 10³ CFU/mL) and Clostridium perfringens (1.0 x 10⁴ CFU/mL). Repeatability was acceptable, with relative standard deviation values ranging from 10% to 35%. Heat treatment increased RLU values, confirming the detection of total adenylate following cell disruption. Overall, the A3 method is suitable as a rapid screening tool for surface hygiene monitoring, although it does not replace conventional microbiological methods for confirmatory analysis.