Development of a procedure for quantifying flavonoids in Hibiscus rosa-sinensis l. tea bags using spectrophotometric method

Abstract

    According to research, flavonoids are known to be a component with a high proportion in Hibiscus rosa-sinensis L. flower extract. Therefore, the present study was performed to evaluate the flavonoids content in Hibiscus rosa-sinensis L. tea bags, by using spectrophotometric method. The study results indicate that the total flavonoid content in Hibiscus rosa-sinensis L. tea bags varies depending on extraction conditions and analytical methods. The ELISA-based method generally yielded higher values than the UV-Vis method. The highest flavonoid content was observed with water extraction at 90 °C (3.04 mgQE/g by ELISA and 2.83 mgQE/g by UV-Vis). The RSD values for the ELISA (0.05%) and UV-Vis (0.2%) methods were both below 2%, demonstrating good precision. The analytical procedure was validated and met all required criteria for the analysis of Hibiscus rosa-sinensis L. tea bags samples. Findings from this study provided essential platform for selecting the conditions for Hibiscus rosa-sinensis L. tea bags extraction, thereby ensuring product quality, increasing economic value, utilizing available flower resources while suggesting optimal conditions for rapid detection of flavonoid content.