INVESTIGATION OF POLYPHENOL, FLAVONOID CONTENT, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF LIPPIA NODIFLORA LEAVES (VERBENACEAE)

Abstract

The species Lippia nodiflora, commonly known as the frog fruit, is a plant species belonging to the Verbenaceae family. It has long been used in traditional medicine
to enhance immunity, alleviate allergic skin reactions, as well as treat acne. Three crude extracts from Lippia nodiflora were prepared using solvents with increasing polarity
(n-hexan, ethyl acetate, and methanol). The total flavonoid and polyphenol content as well as the antioxidant and antimicrobial activities of the three extracts were
investigated in this rearch. The flavonoid content of methanol, ethyl acetate, and n-hexan extracts were found to be 96.11, 126.15, and 77.98mg GAE/g, respectively. The
total polyphenol content were 69.26, 37.68, and 27.09mg QE/g, respectively. The EC50 values for the reducing power of methanol and ethyl acetate extracts were 33.13
and 1514µg/mL, respectively. Additionally, the EC50 values for H2O2 free radical scavenging ability of these extracts were determined to be 163.04, 67.56, and 83.69 µg/mL,
respectively. All three extracts exhibited antimicrobial activity against Bacillus cereus ATCC 11778. Furthermore, the n-hexan extract pointed out the inhibitory effects on
Pseudomonas putida 3326K1 bacteria.