STUDY ON THE EFFECTS OF EXTRACTION METHODS ON CHLOROPHYLL RECOVERY FROM Ceratophyllum submersum ALGAE

Abstract

This study aimed to obtain the total chlorophyll content from Ceratophyllum submersum L. using three extraction methods, including maceration (static soaking), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE). The purpose of the study was to evaluate the effectiveness of these three extraction methods in obtaining chlorophyll from C. submersum. The chlorophyll content of 0.176 mg/g dry weight was achieved by macerating using 80% ethanol as the solvent at 50 °C for 4 hours. The extraction was done using a microwave that assisted with a 1:30 (w/v) material-to-solvent ratio, 360 W, and a 5-minute extraction time, obtaining a chlorophyll content of 0.181 mg/g dry weight. The UAE method required a power of 262.5 W for 20 minutes, resulting in a chlorophyll content of 0.147 mg/g dry weight. According to the results, MAE was superior to both maceration and UAE in obtaining chlorophyll from C. submersum.