Cultivating the black poplar mushroom (cyclocybe aegerita) on a sawdust basal substrate supplemented with rice husk

Abstract

This study was conducted to determine substrate with the optimal supplement proportion of sawdust to rice husk for cultivating the Black Poplar mushroom to achieve the highest biological efficiency. Accordingly, isolated strain was cultivated on four different mixed substrates with varying sawdust-to-rice husk ratios such as 100% sawdust, 90% sawdust: 10% rice husk, 80% sawdust: 20% rice husk, and 70% sawdust: 30% rice husk. On morphological observation and 28S rRNA sequencing, the isolated strain was confirmed to be Cyclocybe aegerita. The result of cultivation C. aegerita on six different culture media (PDA, MEA, GPA, YNA, SDA and Hansen) showed that the GPA medium was optimal, with a mycelial growth rate of 0.76 cm/day, characterized mycelium as dense, uniform, and white mycelium. Among the tested substrates, the substrate consisting of 70% sawdust and 30 rice husk was most suitable for cultivating C. aegerita, achieving a biological efficiency of 34.96%. On this substrate, the mycelial growth rate (0.27 cm/day) was the fastest, the time to fruiting body formation was 17.2 days, and the fresh weight of fruiting body was 97.85 g/bag.