In vitro propagation of Gerbera (Gerbera jamesonii) using the thin cell layer technique at Kien Giang University

Abstract

Gerbera (Gerbera jamesonii) belongs to the Asteraceae. It is one of the most popular ornamental flowers worldwide and used both as cut flower and potted plant. They have some characters such as color, floral diameter, stem length, and vigor, which make this plant of commercial importance. Therefore, Gerbera tissue culture is essential to provide seedling for production. The purpose of this study was to observe the influence of plant growth regulators on in vitro propagation of Gerbera. The results showed that: i) all the samples had formed callus after 21 days of culture and the best callus formation obtained when the thin cell layer of Gerbera was treated with 0,5-0,5, and 0,5-1 mg/l of NAA – BAP; ii) the best medium for shoot formation from callus was MS1 (8,5 shoots per explant), the best medium for shoot formation from callus was MS1 (8,5 shoots per explant), thus reducing costs and inhibitory effect of high concentrations of macronutrients; iii) MS1 medium supplemented with 1 mg/l kinetin was the best for shoot multiplication (5 shoots per explant); iv) MS1 medium supplemented with 1 mg/l NAA was the best for root and plantlet formation.