Effects of Kinetin, NAA, and IBA concentration on in vitro shoot proliferation and root induction of Philodendron billietiae

Abstract

The study on the effects of Kinetin, NAA, and IBA on in vitro shoot multiplication and root formation of Philodendron billietiae was conducted to determine the suitable basal MS media supplemented with concentrations of Kinetin, NAA and IBA for in vitro culture. The experiments were carried out at the Tissue and Cell Technology Lab, College of Agriculture, Can Tho University, comprised of three experiments: The effect of Kinetin and NAA concentrations on the in vitro shoot regeneration stage; The effect of IBA concentration on the in vitro root formation of P. billietiae; and Observation on the survival rate of P. billietiae plantlets under nursery conditions. The results showed that: (i) During the shoot multiplication stage, MS medium supplemented with Kinetin 2 mg/L and NAA 0.1 mg/L yielded the best results at 10 weeks after cultured (3.61 shoots per explant); (ii) MS medium supplemented with 2 mg/L IBA resulted in a 100% root formation rate at 6 weeks after cultured; (iii) During the acclimatization phase, P. billietiae plantlets showed a high survival rate (100%) under both covered and uncovered with plastic bag. The plantlets adapted and developed well after 4 weeks acclimatized and could be transferred to natural condition.