Study of culture medium for organic–oriented Cordyceps militaris
Abstract
The study aimed to determine the isolation and propagation medium for level 1, level 2 (liquid culture) of medicinal mushrooms (Cordyceps militaris) in an organic way. For the isolation medium of Cordyceps militaris, the mushroom tissue was cut from the fruiting body of the mushroom that was disease-free, well-growing, and cultured on a PDA medium. The sample was sterilized by 70% alcohol for 20 seconds, resulting in thick and uniform growth of mycelium, and no infection. For the primary propagation medium of Cordyceps militaris, the experiment used medium: 30g glucose + 3g soybean pepton + 3g yeast extract + 3g silkworm pupae + 20g agar per 1 liter of lip field for optimal results. The mycelium grew uniformly and had a white color, and mycelium grew symmetrically from the location of the original seedling to the primary seed medium. The level 2 propagation medium (liquid culture) was: 30g glucose + 4g Soy Pepton + 4g yeast extract + 4g silkworm pupae per 1 liter of medium for optimal results. The results obtained small fungal cocoons with a diameter of 2mm, uniform in the environment, and the density of the bridge was 8-10 bridges/1cm3.
