ISOLATION AND IDENTIFICATION OF POLY- β -HYDROXYBUTYRATE BIOSYNTHESIS FROM BOTANICAL RESOURCES IN BINH DUONG PROVINCE, VIETNAM

Abstract

Bioplastic poly-β-hydroxybutyrate (PHB) derived from bacteria is known as a
substance with high thermal stability and ready biodegradability. The aim of this study was to isolate and identify some bacteria that would be able to synthesize plastic by biological pathway from soil and botanical resources in Binh Duong province, Vietnam. Optimization of incubation time periods was conducted to maximize PHB accumulation. The results of 16S rRNA sequencing indicated the bacterial isolates exhibited PHB synthesis are Rhizobium gallicum and Agrobacterium tuminfaciens. The highest PHB yield was 39,84% obtained when Rhizobium gallicum was cultured in 48 hours period. The FTIR spectrum of
bioplastic sample showed the similarity in its characteristics to the commercial PHB. The present study has thus provided useful data on some bacterial isolates for PHB production that can be potential for industrial application in manufacture of PHB, an emerging alternative of non-biodegradable plastics.

Keywords: Poly-β–hydroxybutyrate, PHB, Rhizobium gallicum, Agrobacterium tuminfaciens, bioplastic