DESIGN PCR PRIMER FOR DISTINGUISHING BETWEEN Panax stipuleanatus AND Panax notoginseng

Abstract

Panax is a precious genus in the family of Araliaceae family, most species of this genus have high economic value due to their medicinal properties. Compared to other species, Panax stipuleanatus and Panax notoginseng have not been intensively studied. Furthermore, the idenfication of these species is mainly based on morphology and biochemistry leading to low differentiation results if the sample has been preliminary treated or undergoing poor preservation condition. This study focused on the design of a specific primer pair and the development of a PCR protocol to distinguish between Panax stipuleanatus and Panax notoginseng. The obtained primer and PCR protocol showed capacity to accurately distinguish these two Panax species. PCR products were then sequenced and compared with those on NCBI Genbank to confirm the accuracy developed PCR reactions. The results could be applicable in the identification and classification of medicinal materials derived from Panax stipuleanatus and Panax notoginseng.