INVESTIGATION OF FACTORS AFFECTING THE EXTRACTION OF TRITERPENOIDS FROM Ganoderma lucidum BY ULTRASOUND-ASSISTED ENZYMATIC EXTRACTION

Abstract

Triterpenoids in Ganoderma lucidum (G. lucidum) are known for their outstanding biological activities such as antioxidant, antibacterial, anticancer, and antidiabetes. The aim of this study was to provide suitable conditions to obtain the highest-triterpenoid G. lucidum by ultrasonic-assisted enzyme extraction (UAEE) method. Therefore, the study investigated the conditions affecting the extraction process such as enzyme concentration (%), solvent: material ratio (mL/g), temperature (oC), extraction time (hour), and ultrasonic power (W), according to the single-factor experiment. The results showed that the obtained triterpenoid content was 11.33 mg/g of dry matter with enzyme concentration of 2.5 %, solvent: material ratio of 20:1 mL/g, the temperature of 60 oC, 60 min of ultrasonic time, and ultrasonic power of 480 W. The free radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl of the extract with an IC50 value of 379.8 µg/mL. The UAEE method is considered a green approach in extracting high-quality triterpenoid mushrooms, suitable for applications in pharmaceutical technology and functional foods.