RESEARCH ON MICROPROPAGATION PROCEDURE OF Philodendron ‘Jungle boogie’

Abstract

This study aimed to determine the effects of the concentration of plant growth regulators on shoots, rooting and the effects of organic additives on the growth of Philodendron sp. The shoots were cultured on MS media supplemented with separate additional benzyl adenine (BA), kinetin and thidiazuron (TDZ) at different concentrations. After 12 weeks of culture, the results show that the significantly highest number of shoots was found on medium supplemented with BAP at 1.0 mg/L, with 59 shoots/sample. The supplementation of coconut water and potatoes was investigated to find the most suitable organic additives for shoot growth. After 6 weeks of culture, the best growth was obtained with MS media supplemented with 100 mL/L of coconut water. MS media free of naphthalene acid acetic (NAA) was the most appropriate medium for rooting. Following the in vitro multiplication and rooting step, in vitro shoots were cultured in closed vessels and ventilation plastic bags. Plastic bags resulted in better shoot growth, and the survival rate at the nursery stage was 73.33%.