OPTIMIZATION CONDITIONS FOR EXTRACTION OF SAPONIN TRITERPENOID FROM Camellia oleifera BY SURFACE RESPONSE METHOD (RSM)

Abstract

The study aimed to investigate some of factors affecting the extraction of triterpenoid saponins from the residue of seeds of Camellia oleifera by ethanol solvent. The influence of factors including solvent concentration (ethanol), material/solvent ratio, and extraction time were investigated in turn. The content of triterpenoid saponins was determined by UV-Vis spectrophotometric method after sample extraction and rosenthaler coloration reaction of saponins with reagents of perchloric acid and vanillin in acetic acid gave the product a lilac purple color. Optimization of extraction conditions was performed by Response Surface Method (RSM) combined with the complex center model (CCD). The residue of seeds of Camellia oleifera was purchased from the local oil press at Quang Thanh facility, Nghe An province, then dried at 105 ºC for 5 hours, was used for the experiments. According to the CCD model, the optimal results showed that the triterpenoid saponin content 5.451% at the liquid-solid ratio 19: 1 (v/w), the EtOH concentration 80%, and the time 77 hours. The experimental results show high compatibility with the model