EFFECT OF PHYTOHORMONES ON IN-VITRO CALLUS FORMATION FROM LEAVES AND STEMS OF LAVENDER (Lavandula dentata)

Abstract

Formations and morphogenesis in callus were investigated in Lavandula dentata. Callus was obtained from different parts of plants such as leaves and stems cultured on MS medium (Murashige & Skoog) supplemented with NAA (Naphthylacetic acid) and BA (Benzyl adenine). After 60 days of in-vitro culture, leaves culturing in MS medium with NAA 0.1 mg/L and BA 2.0 mg/L was most suitable for callus formation leading to shoot buds multiplication due to these calluses. On the other hand, MS medium supplemented with NAA 1.0 mg/L and BA 2.0 mg/L was also used to give callus which was 100% callus formation. Stem in-vitro was used as callus sources to culture on MS medium having phytohormones, the best result was 100% callus formation in MS medium with NAA 2.0 mg/L and BA 1.0 mg/L which allowed shoot buds to multiply.

Keywords: BA, NAA, callus, in-vitro, Lavender (Lavandula dentata).