THE STUDY OF THE AFFECTION OF PLANT GROWTH REGULATORS ON SHOOT MULTIPLICATION OF ACACIA HYBRID BV75 SUBSPECIES BY IN VITRO METHOD

Abstract

With the outstanding advantages of Acacia hybrid compared to other forest species, this species currently occupies a large part of Vietnam's afforestation area. However, propagation by traditional methods such as cuttings and seeding does not meet the demand for disease-free seedlings, uniform in quality. Breeding technology by tissue culture method is considered the leading technology solution to maintain the genetic quality of seedlings. Plant Growth Regulators are an important factor in increasing shoot multiplier. Cytokinin N6-benzyladenine (BAP) has the ability to stimulate the Acacia hybrid BV75subspecies shoot buds with high shoot multiplier (7.7 shoots/clump, shoot multiplier is 2.96 times) at 1.5mg/L in MS * media. When using Cytokinin and Auxin combination, the most suitable combination for multiplication is 1.5mg/L BAP and 1.0mg/L NAA in MS * medium (7.8 buds/clump and multiplier buds 3.0 times, bud height is 4.5 cm, buds grow well). This is also the environment for the best quality shoots to serve the next phase of research, rooting.