Determination of Aflatoxin B1 in cereal for infants using ultra-performance liquid chromatography to triple quadrupole mass (UPLC-MS/MS) cleaning up by an immunoaffinity column

Abstract

This work studied on developing the analytical procedure for determination of Aflatoxin B1 (AFB1) in cereal for infants by using ultra-performance liquid chromatography triple quadrupole mass spectrometry detector (UPLC-MS/MS). Under optimal conditions, the retention time of AFB1 is identified at 4.07 minutes as using BEH C18 1,7 µm (2,1´150 mm) column and mobile phase with two solutions gradient: 5mM Ammonium acetate/H₂O and 0,1% HCOOH/MeOH, and ratio methanol/water is 70/30 for extraction. The mass-to-charge ratio (m/z) of AFB1 for quantitative and qualitative analyses are 313.05>285 and 313.05>241, respectively. Furthermore, the obtained results are validated. Typically, the statistical parameters are significant such as linear correlation coefficient of determination (R²) ≥ 0.9993, repeatability (concentration ≤ 1µg/kg) in range of (6.72 - 9.00) %, (7.89 - 10.4) % of reproducibility and uncertainty is ±17.1%, while the limit of detection (LOD) and the limit of quantitation (LOQ) are 0.03 µg/kg and 0.10 µg/kg, respectively.