Identification and characterization of an ectoine producing bacterium strain VK124 isolated from mangrove soil samples

Abstract

  In this study, about 200 bacterial colonies were isolated from mangrove soil samples collected from Giao Thuy district, Nam Dinh province. Five colonies were randomly chosen for the evaluation of ectoine production. Among them, four strains can produce ectoine; the highest ectoine producer (strain VK124) was selected for further study. The effect of different media and NaCl concentrations on bacterial cell growth and ectoine accumulation was investigated. Maximum ectoine content of 13.5% was obtained when strain VK124 grown on HM medium containing 15% NaCl. The sequence of 16S rDNA gene and some biochemical characteristics of strain VK124 were also analysed. The results showed that the bacterial strain VK124 belongs to genus Yangia and named as Yangia sp. VK124.