Expression and purificaton of a recombinant phytase originated from Bacillus subtilis in batch cultivation of escherichia coli

Abstract

   Phytase is an important enzyme in feed and food processing. In this study, common medium (LB broth) have been modified for batch cultivation of E. coli BL21(DE3) to express phyC gene coding for a recombinant phytase (BacP) originated from Bacillus suntilis. Recombinant BacP enzyme was recovered at 18.49 IU/ml after 18 hours of induction by lactose, which was higher than that of induction by isopropyl β-D-1-thio-galactopyranoside (IPTG). Cutivation both was concentrated by filteration through a 5 KDa cutoff membrane and BacP enzyme was purified 4.5 times by affinity chromatography through Ni-NTA column to reach a specific activity of 24,48 IU/mg protein. These shall be a preliminary results for further study on application of phytase enzyme BacP which can catalyse the hydrolysis of phytate to lower myo- inositol phosphate (IP2 and IP3) having potential health benefit in food.